丁建平
丁建平： 安徽怀宁县人，男，1964年生，学士，副教授，主要从事动物生理学教学和研究工作，参与完成多项安徽省和合肥市重点科研项目，获得安徽省科技进步一等奖和多项研究成果，发表论文20余篇。技术专长：山羊养殖和胚胎生物技术。
上海生科院生化细胞所研究员个人简介：
1982年1月南京大学获理学学士学位；1984年10月中山大学获理学硕士学位；1987年10月复旦大学获理学博士学位。1987年11月至1989年6月，中国科学院上海硅酸盐研究所博士后。1989年7月至1991年8月，联邦德国柏林自由大学结晶学研究所洪堡基金会奖研金学者。1991年9月至2001年1月，美国新泽西州罗格斯大学，先后任化学与化学生物学系研究助理、研究助理教授、研究副教授，以及高级生物技术和医学研究中心助理研究员和研究员。2000年11月起，任中科院上海生科院生化细胞所研究员，为中科院“百人计划”引进的杰出青年学术带头人，获得2001年国家杰出青年基金资助，2006年入选国家人事部“新世纪百千万人才工程”国家级人选。现任生化细胞所学术委员会副主任，分子生物学国家重点实验室副主任，“中国生物化学与分子生物学学会”理事、“蛋白质组学专业委员会”副主任委员、“蛋白质科学专业委员会” 常务理事；“中国生物物理学会”理事；“中国晶体学会”常务理事；“亚洲晶体学会”常务理事等；中国《生物化学与生物物理学报》、《生物化学与生物物理进展》和《生命的化学》编委、英国《The Biochemical Journal》编辑顾问委员会委员等
研究方向：染色质修饰蛋白质的结构生物学
研究工作：
研究领域为结构生物学，研究工作主要是运用蛋白质结晶学、分子生物学、生物化学、细胞生物学等方法，研究生物大分子的结构和功能。目前研究工作主要围绕一些具有重要生物学意义、并且与重要疾病相关的人源蛋白质的结构和功能进行研究，特别是参与染色质修饰的蛋白质、参与营养和能量调节的mTOR信号转导通路的蛋白质、以及参与合成和分解代谢过程的重要酶蛋白等。近五年来主持、参与并完成国家、科学院和地方政府的多项重大研究项目。现已在国际重要学术刊物上发表研究论文100多篇，其中包括Nature, Nature Struct Biol, PNAS, EMBO J, J Biol Chem, Nucleic Acid Res, Structure, J Mol Biol, Biochem J, Virology等。这些研究工作不仅阐述了一些重要蛋白质分子的生物功能的结构基础、反应机理、结构和功能的关系等一系列科学问题，具有重大的科学意义；同时还为探索与这些蛋白的功能失调相关的疾病的发病机理、寻找疾病诊断的新靶标和新方法、设计和开发抗病毒和抗疾病药物等奠定了分子基础，具有重要的潜在应用价值。
发表论文：
Du J, Hou S, Zhong C, Lai Z, Yang H, Dai J, Zhang D, Wang H, Guo Y, Ding J*. Molecular basis of recognition of human osteopontin by 23C3, a potential therapeutic antibody for treatment of rheumatoid arthritis. J Mol Biol, doi:10.1016/j.jmb.2008.07.075, Epub Aug. 4 (2008).
Ma J, Zhang P, Zhang Z, Zha M, Xu H, Zhao G, Ding J*. Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans. Biochem J, doi:10.1042/BJ20080242, Epub May 23 (2008).
Peng Y, Zhong C, Huang W, Ding J*. Structural studies of Saccharomyces cerevesiae mitochondrial NADP-dependent isocitrate dehydrogenase in different enzymatic states reveal substantial conformational changes during the catalytic reaction. Protein Sci, 2008 Jun 13;17(9):1542-1554.
Hu T, Wu D, Chen J, Ding J, Jiang H, Shen X. The catalytic intermediate stabilized by a "down" active site loop for diaminopimelate decarboxylase from helicobacter pylori: Enzymatic characterization with crystal structure analysis. J Biol Chem, 2008 Jul 25;283(30):21284-21293.
Yu XL$, Hu T$, Du J$, Ding J$, Yang XM, Zhang J, Yang B, Shen X, Zhang Z, Zhong WD, Wen N, Jiang H, Zhu P, Chen ZN. Crystal structure of HAb18G/CD147: implications for immunoglobulin superfamily homophilic adhesion. J Biol Chem, 283, 18056-18065 (2008). ($ These authors contributed equally.)
Wu D, Hu T, Zhang L, Chen J, Du J, Ding J, Jiang H, Shen X. Residues Asp164 and Glu165 at the substrate entryway function potently in substrate orientation of alanine racemase from E. coli: Enzymatic characterization with crystal structure analysis. Protein Sci, 17, 1066-1076 (2008).
Zha M, Guo Q, Zhang Y, Yu B, Ou Y, Zhong C, Ding J*. Molecular mechanism of ADPR hydrolysis by human NUDT5 from structural and kinetic studies. J Mol Biol. 2008 Jun 6;379(3):568-78.
Du J, Wang H, Zhong C, Peng B, Zhang M, Li B, Huo S, Guo Y, Ding J*. Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide. Mol Immunol. 2008 May;45(10):2861-8.
Dalei Wu, Liang Zhang, Yunhua Kong, Jiamu Du, Shuai Chen, Jing Chen, Jianping Ding, Hualiang Jiang*, Xu Shen. Enzymatic characterization and crystal structure analysis of the D-alanine ligase from Helicobacter pylori (p NA). Proteins, 2008 Mar;72(4):1148-1160.
Shen N, Zhou M, Yang B, Yu Y, Dong X, Ding J*. Catalytic mechanism of the tryptophan activation reaction revealed by crystal structures of human tryptophanyl-tRNA synthetase in different enzymatic states. Nucleic Acids Res. 2008 Jan; 36(4):1288-1299.
Shuai Chen, Tiancen Hu, Jian Zhang, Jing Chen, Kaixian Chen, Jianping Ding, Hualiang Jiang, Xu Shen. Mutation of Gly11 on the dimer interface results in the complete crystallographic dimer dissociation of SARS-CoV 3CLpro: Crystal structure with molecular dynamics simulations. J Biol Chem. 2008;283, 554-564.
Wu J, Tao Y, Zhang M, Howard M, Gutteridge S, Ding J*. Crystal structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoyl-CoA and inhibitors reveal the functional roles of the N-terminal region. J Biol Chem. 2007;282, 22185-22194.
Du J, Wang H, Zhong C, Peng B, Zhang M, Li B, Huo S, Guo Y, Ding J. Structural basis for recognition of CD20 by therapeutic antibody Rituximab. J Biol Chem. 2007 May 18;282(20):15073-15080.
Yongxiang Wang, Xiang Xu, Cheng Luo, Zhangmei Ma, Hualiang Jiang, Jianping Ding, Yumei Wen. A putative new domain target for anti-hepatitis B virus: Residues flanking hepatitis B virus reverse transcriptase residue 306 (rtP306). J Med Virol. 2007 Apr;79(6):676-82.
Wang Yongxiang, Xiang Xu, Cheng Luo, Ma Zhangmei, Jiang Hualiang, Ding Jianping, Wen Yumei. Mutational analysis revealed that conservation of hepatitis B virus reverse transcriptase residue 306 (rtP306) is crucial for encapsidation of pregenomic RNA. FEBS Lett. 2007;581(3):558-64.
Li S, Lu Y, Peng B, Ding J*. Crystal structure of human phosphoribosylpyro-phosphate synthetase 1 reveals a new allosteric site. Biochem J. 2007;401, 39-47.
Zhang P, Du J, Sun B, Dong X, Xu G, Zhou J, Huang Q, Liu Q, Hao Q, Ding J*. Structure of human MRG15 chromo domain and its binding to Lys36-methylated histone H3. Nucleic Acids Res. 2006 Nov;34(22):6621-6628.
Zha M, Zhong C, Peng Y, Ding J*. Crystal structures of human NUDT5 reveal the structural basis of the substrate specificity. J Mol Biol. 2006 Dec 15;364(5):1021-1033.
Zhang P, Zhao J, Wang B, Du J, Chen J, Ding J*. The MRG domain of human MRG15 uses a shallow hydrophobic pocket to interact with the N-terminal region of PAM14. Protein Science, 2006 Oct;15(10):2423-2434.
Cai J, Han C, Hu T, Zhang J, Wu D, Wang F, Liu Y, Ding J, Chen K, Yue J, Shen X, Jiang H. Peptide deformylase is a potential target for anti-Helicobacter pylori drugs: Reverse docking, enzymatic assay, and X-ray crystallography validation. Protein Science, 2006 Sep;15(9):2071-2081.
Shen N, Guo L, Yang B, Jin Y, Ding J*. Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity. Nucleic Acids Res. 2006 Jun 23;34(11):3246-58.
Xie ZH, Huang YN, Chen ZX, Riggs AD, Ding JP, Gowher H, Jeltsch A, Sasaki H, Hata K, Xu GL. Mutations in DNA methyltransferase DNMT3B in ICF syndrome affects its regulation by DNMT3L. Hum Mol Genet. 2006 May 1;15(9):1375-85.
Maeda K, Das D, Ogata-Aoki H, Nakata H, Miyakawa T, Tojo Y, Norman R, Takaoka Y, Ding J, Arnold E, Mitsuya H. Structural and molecular interactions of CCR5 inhibitors with CCR5. J Biol Chem. 2006 May 5;281(18):12688-12698.
Liu Y, Liao J, Zhu B, Wang ED, Ding J. Crystal structures of the editing domain of Escherichia coli leucyl-tRNA synthetase and its complexes with Met and Ile reveal a lock-and-key mechanism for amino acid discrimination. Biochem J. 2006 Mar 1;394(Pt 2):399-407.
Yu Y, Li S, Xu X, Li Y, Guan K, Arnold E, Ding J. Structural basis for the unique biological function of small GTPase RHEB. J Biol Chem. 2005 Apr 29;280(17):17093-100.
Li J, Xia Z, Ding J*. Thioredocin-like domain of human kappa class transferase reveals sequence homology and structure similarity to the theta class enzyme. Protein Science, 2005 Sep;14(9):2361-2369.
Cheng A, Zhang W, Xie Y, Jiang W, Arnold E, Sarafianos SG, Ding J. Expression, purification, and characterization of SARS coronavirus RNA polymerase. Virology. 2005 May 10;335(2):165-76.
Lin X, Ma ZM, Yao X, He LF, Yuan ZH, Ding JP, Wen YM. Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication. J Gen Virol. 2005 Jan;86(Pt 1):85-90.
Tuske S, Sarafianos SG, Clark AD Jr, Ding J, Naeger LK, White KL, Miller MD, Gibbs CS, Boyer PL, Clark P, Wang G, Gaffney BL, Jones RA, Jerina DM, Hughes SH, Arnold E. Structures of HIV-1 RT-DNA complexes before and after incorporation of the anti-AIDS drug tenofovir. Nat Struct Mol Biol. 2004 May;11(5):469-74.
Yu Y, Liu Y, Shen N, Xu Z, Xu F, Jia J, Jin Y, Arnold E, Ding J*. Crystal structure of human tryptophanyl-tRNA synthetase catalytic fragment at 2.5 Å resolution: insights into substrate recognition, tRNA binding, and angiogenesis activity. J Biol Chem. 2004 Feb;279(9):8378-8388.
Xu X, Zhao J, Xu Z, Peng B, Huang Q, Arnold E, Ding J. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. J Biol Chem. 2004 Aug 6;279(32):33946-57.
Xu PL, Liu YQ, Shan SF, Kong YY, Zhou Q, Li M, Ding JP, Xie YH, Wang Y. Molecular mechanism for the potentiation of the transcriptional activity of human liver receptor homolog 1 by steroid receptor coactivator-1. Mol Endocrinol. 2004 Aug;18(8):1887-905.
Yu Y, Chang Y, Li S, Hu H, Huang Q, Ding J. Expression, purification, crystallization and preliminary structural characterization of the GTPase domain of human Rheb. Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1883-7.
Ge YZ, Pu MT, Gowher H, Wu HP, Ding JP, Jeltsch A, Xu GL. Chromatin targeting of de novo DNA methyltransferases by the PWWP domain. J Biol Chem. 2004 Jun 11;279(24):25447-54.
Jia J, Chen XL, Guo LT, Yu YD, Ding JP, Jin YX. Residues K149 and E153 switch the aminoacylation of tRNATrp in Bacillus subtilis. J Biol Chem. 2004 Oct 1;279, 41960-41965.
Gan X, Ma Z, Deng N, Wang J, Ding J, Li L. Involvement of the C-terminal proline-rich motif of G protein-coupled receptor kinases in recognition of activated rhodopsin. J Biol Chem. 2004 Nov 26;279(48):49741-6.
Yu K, Fu W, Liu H, Luo X, Chen KX, Ding J, Shen J, Jiang H. Computational simulations of interactions of scorpion toxins with the voltage-gated potassium ion channel. Biophys J. 2004 Jun;86(6):3542-55.
Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, Ding J*. Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Research, 2003;31, 7117-7130.
Xu Z, Liu Y, Yang Y, Jiang W, Arnold E, Ding J*. Crystal structure of D-hydantoinase from Burkholderia pickettii at 2.7 Å resolution: insights into the molecular determinants of enzyme thermostability. J Bacteriology, 2003;185, 4038-4049.
Shen L, Shen J, Luo X, Cheng F, Xu Y, Chen K, Arnold E, Ding J*, Jiang H*. Steered molecular dynamics simulation on the binding of NNRTI to HIV-1 RT. Biophys J. 2003;84, 3547-3563.
河北医科大学第三医院教授河北医科大学第三医院医学影像科副主任、主任医师、教授、硕士生导师。曾获河北医科大学硕士学位、北京大学医学博士学位，1999年至2000年于日本信州大学医学部附属病院放射线科做访问学者。研究方向为骨关节系统和泌尿系统影像诊断。先后在本专业《中华放射学杂志》等核心杂志发表论文四十余篇。主编人民卫生出版社出版的《骨关节x线片读片指导》等视听教材两部，参编人民卫生出版社出版的《中华影像医学一骨肌系统卷》、《临床医学问答》等著作4部。曾获河北省卫生厅科技进步一等奖、二等奖和河北省科技厅科技进步三等奖多次，还获得白求恩式先进工作者、优秀教师、先进科技工作者等荣誉。现承担河北省科技厅科研课题2项。