BCA

英文全称: Business-Critical Applications

中文译名: 企业要害应用

分 类: IP与多媒体

解 释: 为了运行一个企业所需的极重要软件，不管是专门编写的还是商用化打包的，如会计/财务、企业资源规划（ERP）、制造、人力资源和销售数据库。

BCA assay Bicinchoninic acid assay

The bicinchoninic acid assay or BCA assay is a biochemical assay for determining the total level of protein in a solution, similar to Lowry protein assay, Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

Mechanism

A stock BCA solution contains the following ingredients in a highly alkaline solution with a pH 11.25:

Bicinchoninic acid

Sodium carbonate

Sodium bicarbonate

Sodium tartrate

Cupric sulfate pentahydrate

The BCA assay relies on two reactions. First, the peptide bonds in protein reduce Cu2+ ions from the cupric sulfate to Cu1+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid chelate with each Cu1+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm.

The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations.